A compendium of next-generation patient-derived models for diverse cancers

ElHarouni, Dina, Al-Jazrawe, Mushriq, Choi, Seongmin, Dede, Merve, Hinoue, Toshinori, Misek, Sean A, Noh, Heeju, Zanella, Luca, Tseng, Yuen-Yi, Francies, Hayley E, Plenker, Dennis, Kyi, Cindy W, Perez-Mayoral, Julyann, Stine, Megan J, Tonsing-Carter, Eva, Agarwal, Rachana, Zenklusen, Jean Claude, Clinton, James M, Shelton, Jennifer M, Chu, Timothy R, Hooper, William F, Loinaz, Xavi, Keskula, Paula, Tagle, Jordan, Kuhlers, Peyton C, Tercan, Bahar, Boj, Sylvia F, Vasciaveo, Alessandro, Tomassoni, Lorenzo, Crawford, James M, Walsh, Shawna, Sinai, Claire, Bhatia, Sonam, Sridevi, Priya, Patel, Hardik, Cerone, Maria Antonietta, HCMI Network, Ellrott, Kyle, Kuo, Calvin J, Elemento, Olivier, Beyaz, Semir, Corbo, Vincenzo, Spector, David L, Beroukhim, Rameen, Ferguson, Martin L, Cherniack, Andrew D, Laird, Peter W, Robine, Nicolas, McPherson, Andrew, Hoadley, Katherine A, Garnett, Mathew J, Tuveson, David A, Califano, Andrea, Spellman, Paul T, Ligon, Keith L, Gerhard, Daniela S, Staudt, Louis M, Boehm, Jesse S (August 2026) A compendium of next-generation patient-derived models for diverse cancers. Nature. ISSN 0028-0836

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Abstract

The development of new therapeutics and the validation of pathogenetic cancer mechanisms require representative laboratory models1,2. However, existing collections represent only a fraction of the diversity observed in human cancer2-4. Recent technologies have enabled efficient in vitro model derivation (for example, tumour organoids)5. However, whether these maintain essential properties of patient tumours during long-term expansion has not been systematically investigated. Here we present results of a large-scale international programme-the Human Cancer Models Initiative-which involved the generation of a resource of 665 next-generation models from 2,780 donors with 25 cancer types and integrated tumour-model whole genome, exome, methylome and transcriptome analyses. The resource provides 522 models with comprehensive clinical data, 153 models of rare cancers and 71 models from participants with non-European ancestry. Analyses of 421 matched tumour-model pairs reveal high genetic (97.8%) and epigenetic (95%) concordance and define correlates of model discordance. Single-nucleus RNA sequencing of tumour-model pairs reveals subsets of models in which culture conditions significantly influence cell states. Finally, we characterize model preservation of extrachromosomal DNA and post-treatment mutational signatures to provide opportunities to study therapeutic resistance. This model repository is being made available to the community-including multimodal molecular profiling, clinical information and integrative software tools-thus providing a valuable resource for preclinical investigation of cancer pathogenesis and treatment response.

Item Type: Paper
Subjects: diseases & disorders > cancer
diseases & disorders
CSHL Authors:
Communities: CSHL Cancer Center Program > Cancer Genetics and Genomics Program
CSHL Cancer Center Program > Gene Regulation and Inheritance Program
CSHL labs > Beyaz lab
CSHL labs > Krasnitz lab
CSHL labs > McCombie lab
CSHL labs > Spector lab
CSHL labs > Tuveson lab
CSHL Cancer Center Program
CSHL Cancer Center Program > Cellular Communication in Cancer Program
CSHL Post Doctoral Fellows
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 5 August 2026
Date Deposited: 07 Aug 2026 14:50
Last Modified: 07 Aug 2026 14:50
Related URLs:
URI: https://repository.cshl.edu/id/eprint/42285

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