Magnetically Functionalized Hydrogels for High-Throughput Genomic Applications

Lammertse, E, Li, S, Kendall, J, Kim, C, Morris, P, Ranade, N, Levy, D, Wigler, M, Brouzes, E (January 2023) Magnetically Functionalized Hydrogels for High-Throughput Genomic Applications. Advanced Materials Technologies. ISSN 2365-709X

Abstract

Single-cell genomics has revolutionized tissue analysis by revealing the genetic program of individual cells. The key aspect of the technology is the use of barcoded beads to unambiguously tag sequences originating from a single cell. The generation of unique barcodes on beads is mainly achieved by split–pooling methods, which are labor-intensive due to repeated washing steps. Toward the automation of the split–pooling method, a simple method to magnetize hydrogel beads is developed. It is shown that these hydrogel beads provide increased yields and washing efficiencies for purification procedures. They are also fully compatible with single-cell sequencing using the BAG-seq workflow. The work opens the automation of the split–pooling technique, which will improve single-cell genomic workflows.

Item Type: Paper
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
CSHL Authors:
Communities: CSHL labs > Levy lab
CSHL labs > Wigler lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 1 January 2023
Date Deposited: 27 Dec 2023 13:41
Last Modified: 03 Jul 2024 16:08
PMCID: PMC11029686
Related URLs:
URI: https://repository.cshl.edu/id/eprint/41362

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