Lammertse, E, Li, S, Kendall, J, Kim, C, Morris, P, Ranade, N, Levy, D, Wigler, M, Brouzes, E (January 2023) Magnetically Functionalized Hydrogels for High-Throughput Genomic Applications. Advanced Materials Technologies. ISSN 2365-709X
Abstract
Single-cell genomics has revolutionized tissue analysis by revealing the genetic program of individual cells. The key aspect of the technology is the use of barcoded beads to unambiguously tag sequences originating from a single cell. The generation of unique barcodes on beads is mainly achieved by split–pooling methods, which are labor-intensive due to repeated washing steps. Toward the automation of the split–pooling method, a simple method to magnetize hydrogel beads is developed. It is shown that these hydrogel beads provide increased yields and washing efficiencies for purification procedures. They are also fully compatible with single-cell sequencing using the BAG-seq workflow. The work opens the automation of the split–pooling technique, which will improve single-cell genomic workflows.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes |
| CSHL Authors: | |
| Communities: | CSHL labs > Levy lab CSHL labs > Wigler lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 1 January 2023 |
| Date Deposited: | 27 Dec 2023 13:41 |
| Last Modified: | 03 Jul 2024 16:08 |
| PMCID: | PMC11029686 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41362 |
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