Pseudouridine guides germline small RNA transport and epigenetic inheritance

Herridge, Rowan P, Dolata, Jakub, Migliori, Valentina, de Santis Alves, Cristiane, Borges, Filipe, Van Ex, Frédéric, Lin, Ann, Bajczyk, Mateusz, Leonardi, Tommaso, Hendrick, Alan, Schorn, Andrea J, Kouzarides, Tony, Martienssen, Robert A (August 2023) Pseudouridine guides germline small RNA transport and epigenetic inheritance. bioRxiv. (Public Dataset)

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Abstract

UNLABELLED: Epigenetic modifications that arise during plant and animal development, such as DNA and histone modification, are mostly reset during gamete formation, but some are inherited from the germline including those marking imprinted genes 1,2 . Small RNAs guide these epigenetic modifications, and some are also inherited by the next generation 3,4 . In C. elegans , inherited small RNA precursors have poly (UG) tails 5 , but how inherited small RNAs are distinguished in other animals and plants is unknown. Pseudouridine (Ψ) is the most abundant RNA modification but has not been explored in small RNAs. Here, we develop novel assays to detect Ψ in short RNA sequences, demonstrating its presence in mouse and Arabidopsis microRNAs and their precursors. We also detect substantial enrichment in germline small RNAs, namely epigenetically activated siRNAs (easiRNAs) in Arabidopsis pollen, and piwi-interacting piRNAs in mouse testis. In pollen, pseudouridylated easiRNAs are localized to sperm cells, and we found that PAUSED/HEN5 (PSD) , the plant homolog of Exportin-t, interacts genetically with Ψ and is required for transport of easiRNAs into sperm cells from the vegetative nucleus. We further show that Exportin-t is required for the triploid block: chromosome dosage-dependent seed lethality that is epigenetically inherited from pollen. Thus, Ψ has a conserved role in marking inherited small RNAs in the germline. ONE-SENTENCE SUMMARY: Pseudouridine marks germline small RNAs in plants and mammals, impacting epigenetic inheritance via nuclear transport.

Item Type:	Paper
Subjects:	organism description > plant > Arabidopsis organism description > animal > C elegans bioinformatics > genomics and proteomics > genetics & nucleic acid processing > epigenetics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > epigenetics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
CSHL Authors:	Herridge, Rowan Borges, Filipe Van Ex, Frederic Lin, Ann Schorn, Andrea Martienssen, Robert A. Dolata, Jakub de Santis Alves Rosa, Cristiane
Communities:	CSHL labs > Martienssen lab CSHL labs > Schorn lab CSHL Cancer Center Program CSHL Cancer Center Program > Gene Regulation and Inheritance Program CSHL Cancer Center Shared Resources > Sequencing Technology & Analysis Service
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	3 August 2023
Date Deposited:	08 Sep 2023 16:14
Last Modified:	09 Feb 2024 14:41
PMCID:	PMC10312437
Related URLs:	Publisher
Dataset ID:	GSE: 229986 GSE: 230359 GSE: 230228 GSE: 222751
URI:	https://repository.cshl.edu/id/eprint/40895

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