High-throughput sequencing of single neuron projections reveals spatial organization in the olfactory cortex

Chen, Yushu, Chen, Xiaoyin, Baserdem, Batuhan, Zhan, Huiqing, Li, Yan, Davis, Martin B, Kebschull, Justus M, Zador, Anthony M, Koulakov, Alexei A, Albeanu, Dinu F (October 2022) High-throughput sequencing of single neuron projections reveals spatial organization in the olfactory cortex. Cell, 185 (22). 4117-4134.e28. ISSN 0092-8674

Abstract

In most sensory modalities, neuronal connectivity reflects behaviorally relevant stimulus features, such as spatial location, orientation, and sound frequency. By contrast, the prevailing view in the olfactory cortex, based on the reconstruction of dozens of neurons, is that connectivity is random. Here, we used high-throughput sequencing-based neuroanatomical techniques to analyze the projections of 5,309 mouse olfactory bulb and 30,433 piriform cortex output neurons at single-cell resolution. Surprisingly, statistical analysis of this much larger dataset revealed that the olfactory cortex connectivity is spatially structured. Single olfactory bulb neurons targeting a particular location along the anterior-posterior axis of piriform cortex also project to matched, functionally distinct, extra-piriform targets. Moreover, single neurons from the targeted piriform locus also project to the same matched extra-piriform targets, forming triadic circuit motifs. Thus, as in other sensory modalities, olfactory information is routed at early stages of processing to functionally diverse targets in a coordinated manner.

Item Type:	Paper
Subjects:	organism description > animal organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions organism description > animal > mammal organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > tissues types and functions > olfactory bulb organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent
CSHL Authors:	Chen, Xiaoyin Baserdem, Batuhan Zhan, Huiqing Davis, Martin Kebschull, Justus Zador, Anthony M. Koulakov, Alexei A. Albeanu, Dinu F. Li, Yan Chen, Yushu
Communities:	CSHL labs > Albeanu lab CSHL labs > Koulakov lab CSHL labs > Zador lab CSHL labs > Banerjee lab CSHL Cancer Center Program CSHL Cancer Center Shared Resources > Sequencing Technology & Analysis Service
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	27 October 2022
Date Deposited:	07 Nov 2022 21:26
Last Modified:	09 Feb 2024 18:34
PMCID:	PMC9681627
URI:	https://repository.cshl.edu/id/eprint/40746

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