BRICseq Bridges Brain-wide Interregional Connectivity to Neural Activity and Gene Expression in Single Animals.

Huang, Longwen, Kebschull, Justus M, Fürth, Daniel, Musall, Simon, Kaufman, Matthew T, Churchland, Anne K, Zador, Anthony M (July 2020) BRICseq Bridges Brain-wide Interregional Connectivity to Neural Activity and Gene Expression in Single Animals. Cell, 182 (1). 177-188.e27. ISSN 0092-8674

URL: https://www.ncbi.nlm.nih.gov/pubmed/32619423

DOI: 10.1016/j.cell.2020.05.029

Abstract

Comprehensive analysis of neuronal networks requires brain-wide measurement of connectivity, activity, and gene expression. Although high-throughput methods are available for mapping brain-wide activity and transcriptomes, comparable methods for mapping region-to-region connectivity remain slow and expensive because they require averaging across hundreds of brains. Here we describe BRICseq (brain-wide individual animal connectome sequencing), which leverages DNA barcoding and sequencing to map connectivity from single individuals in a few weeks and at low cost. Applying BRICseq to the mouse neocortex, we find that region-to-region connectivity provides a simple bridge relating transcriptome to activity: the spatial expression patterns of a few genes predict region-to-region connectivity, and connectivity predicts activity correlations. We also exploited BRICseq to map the mutant BTBR mouse brain, which lacks a corpus callosum, and recapitulated its known connectopathies. BRICseq allows individual laboratories to compare how age, sex, environment, genetics, and species affect neuronal wiring and to integrate these with functional activity and gene expression.

Item Type:	Paper
Subjects:	Investigative techniques and equipment organism description > animal organism description > animal behavior organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions organism description > animal behavior > decision making bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression organism description > animal > mammal organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transcriptomes Investigative techniques and equipment > Whole Brain Circuit Mapping
CSHL Authors:	Huang, Longwen Kebschull, Justus Musall, Simon Kaufman, Matthew Churchland, Anne K. Zador, Anthony M.
Communities:	CSHL labs > Churchland lab CSHL labs > Zador lab School of Biological Sciences > Publications
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	9 July 2020
Date Deposited:	24 May 2021 19:45
Last Modified:	30 Jan 2024 20:56
PMCID:	PMC7771207
URI:	https://repository.cshl.edu/id/eprint/40161

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