Baker, L. A., Tuveson, D. A. (2019) Generation and Culture of Tumor and Metastatic Organoids from Murine Models of Pancreatic Ductal Adenocarcinoma. Methods Mol Biol, 1882. pp. 117-133. ISSN 1064-3745
Abstract
Pancreatic ductal adenocarcinoma (PDA) is a lethal malignancy that is refractory to all current therapies. Research into the mechanisms driving this cancer is the key to developing better diagnostic and treatment options which are urgently needed in the clinic. Genetically engineered mouse models of PDA have been valuable research tools, enabling studies of all stages of PDA progression. However, these models are difficult and time-consuming to breed, and engineering further mutations into these models requires additional time. Recently, organoid cultures of PDA have emerged as alternative models for this disease. Organoids can be rapidly generated from mouse models of PDA and enable genetic and biochemical perturbation of all stages of PDA progression. Here, we describe the generation and propagation of organoid models from PDA tumors and metastases harvested from genetically engineered mouse models.
| Item Type: | Paper |
|---|---|
| Subjects: | diseases & disorders Investigative techniques and equipment diseases & disorders > neoplasms organism description > animal Investigative techniques and equipment > cell culture > cancer organoids Investigative techniques and equipment > cell culture organism description > animal > mammal organism description > animal > mammal > rodent > mouse diseases & disorders > cancer > cancer types > pancreatic cancer organism description > animal > mammal > rodent |
| CSHL Authors: | |
| Communities: | CSHL labs > Tuveson lab CSHL Cancer Center Program > Cellular Communication in Cancer Program |
| Depositing User: | Matthew Dunn |
| Date: | 2019 |
| Date Deposited: | 12 Nov 2018 21:45 |
| Last Modified: | 01 Feb 2024 20:25 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/37358 |
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