Perez-Mancera, P. A., Rust, A. G., van der Weyden, L., Kristiansen, G., Li, A., Sarver, A. L., Silverstein, K. A., Grutzmann, R., Aust, D., Rummele, P., Knosel, T., Herd, C., Stemple, D. L., Kettleborough, R., Brosnan, J. A., Li, A., Morgan, R., Knight, S., Yu, J., Stegeman, S., Collier, L. S., ten Hoeve, J. J., de Ridder, J., Klein, A. P., Goggins, M., Hruban, R. H., Chang, D. K., Biankin, A. V., Grimmond, S. M., Wessels, L. F., Wood, S. A., Iacobuzio-Donahue, C. A., Pilarsky, C., Largaespada, D. A., Adams, D. J., Tuveson, D. A. (June 2012) The deubiquitinase USP9X suppresses pancreatic ductal adenocarcinoma. Nature, 486 (7402). pp. 266-70. ISSN 1476-4687 (Electronic)0028-0836 (Linking)
Abstract
Pancreatic ductal adenocarcinoma (PDA) remains a lethal malignancy despite much progress concerning its molecular characterization. PDA tumours harbour four signature somatic mutations in addition to numerous lower frequency genetic events of uncertain significance. Here we use Sleeping Beauty (SB) transposon-mediated insertional mutagenesis in a mouse model of pancreatic ductal preneoplasia to identify genes that cooperate with oncogenic Kras(G12D) to accelerate tumorigenesis and promote progression. Our screen revealed new candidate genes for PDA and confirmed the importance of many genes and pathways previously implicated in human PDA. The most commonly mutated gene was the X-linked deubiquitinase Usp9x, which was inactivated in over 50% of the tumours. Although previous work had attributed a pro-survival role to USP9X in human neoplasia, we found instead that loss of Usp9x enhances transformation and protects pancreatic cancer cells from anoikis. Clinically, low USP9X protein and messenger RNA expression in PDA correlates with poor survival after surgery, and USP9X levels are inversely associated with metastatic burden in advanced disease. Furthermore, chromatin modulation with trichostatin A or 5-aza-2'-deoxycytidine elevates USP9X expression in human PDA cell lines, indicating a clinical approach for certain patients. The conditional deletion of Usp9x cooperated with Kras(G12D) to accelerate pancreatic tumorigenesis in mice, validating their genetic interaction. We propose that USP9X is a major tumour suppressor gene with prognostic and therapeutic relevance in PDA.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Anoikis/genetics Carcinoma, Pancreatic Ductal/ enzymology/genetics/pathology Cell Line, Tumor Disease Models, Animal Endopeptidases Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Humans Mice Mice, Inbred C57BL Pancreatic Neoplasms/ enzymology/genetics/pathology U937 Cells Ubiquitin Thiolesterase/ genetics/ metabolism |
| Subjects: | bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes diseases & disorders > cancer > cancer types > pancreatic cancer |
| CSHL Authors: | |
| Communities: | CSHL labs > Tuveson lab |
| Depositing User: | Matt Covey |
| Date: | 14 June 2012 |
| Date Deposited: | 23 Jul 2015 14:45 |
| Last Modified: | 23 Jul 2015 14:45 |
| PMCID: | PMC3376394 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31643 |
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