Blasco, M. A., Funk, W., Villeponteau, B., Greider, C. W.
(September 1995)
Functional characterization and developmental regulation of mouse telomerase RNA.
Science, 269 (5228).
pp. 1267-70.
ISSN 0036-8075 (Print)
Abstract
Telomerase synthesizes telomeric DNA repeats onto chromosome ends de novo. The mouse telomerase RNA component was cloned and contained only 65 percent sequence identity with the human telomerase RNA. Alteration of the template region in vivo generated altered telomerase products. The shorter template regions of the mouse and other rodent telomerase RNAs could account for the shorter distribution of products (processivity) generated by the mouse enzyme relative to the human telomerase. Amounts of telomerase RNA increased in immortal cells derived from primary mouse fibroblasts. RNA was detected in all newborn mouse tissues tested but was decreased during postnatal development.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
3T3 Cells
Animals
Animals, Newborn
Base Sequence
Brain/enzymology
Cell Line, Transformed
Cloning, Molecular
DNA Nucleotidylexotransferase/antagonists &
inhibitors/chemistry/genetics/ metabolism
Gene Expression Regulation, Developmental
Humans
Kidney/enzymology
Liver/enzymology
Mice
Molecular Sequence Data
Muridae
Mutagenesis
Oligonucleotides, Antisense/pharmacology
RNA/chemistry/genetics/ metabolism
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Templates, Genetic
Transfection |
| Subjects: |
bioinformatics > genomics and proteomics > Mapping and Rendering > RNA Structure Rendering
organism description > animal
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > animal > mammal > primates > hominids > human
organs, tissues, organelles, cell types and functions > organs types and functions > liver
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations > mutagenesis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase
Investigative techniques and equipment > transfection |
| CSHL Authors: |
|
| Communities: |
CSHL labs |
| Depositing User: |
Jessica Koos
|
| Date: |
1 September 1995 |
| Date Deposited: |
15 Aug 2014 16:40 |
| Last Modified: |
15 Aug 2014 16:40 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/30568 |
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