Filipkowski, R. K., Rydz, M., Berdel, B., Morys, J., Kaczmarek, L. (March 2000) Tactile experience induces c-fos expression in rat barrel cortex. Learning & Memory, 7 (2). pp. 116-122. ISSN 1072-0502
Preview |
PDF (Paper)
Filipkowski Learning & Memory 2000.pdf - Published Version Download (1MB) | Preview |
Abstract
Understanding gene expression that is responsive to sensory stimulation is central to elucidate molecular mechanisms underlying neuronal plasticity. In this study we demonstrate two new methods of stimulating whiskers that provide major sensory input to rat neocortex. In the first paradigm, animals were placed on the top of a cylinder and their vibrissae were brushed by hand, In the second paradigm, animals were placed for a brief period or time into a new, wired cage resulting in vibrissae stimulation when they explored the new environment. Both approaches induced c-Fos expression in barrel cortex corresponding to the stimulated vibrissae, especially in layer IV. Layers II/III and V/VI also showed c-Fos induction, but there were no detectable changes in layer VIb. The majority of c-Fos-expressing cells are probably not inhibitory neurons, because they do not show parvalbumin staining. Both paradigms, in contrast to the previous methods, are simple to use and do not require anesthesia, restraint of animals, or elaborate experimental setups.
| Item Type: | Paper |
|---|---|
| Additional Information: | Learn. Mem. |
| Uncontrolled Keywords: | CALCIUM-BINDING PROTEINS EARLY GENE-EXPRESSION SOMATOSENSORY CORTEX CEREBRAL-CORTEX VISUAL-CORTEX TRANSCRIPTION FACTORS SENSORY STIMULATION TRANSIENT INCREASE NEURONAL-ACTIVITY NERVOUS-SYSTEM |
| Subjects: | organism description > animal behavior organs, tissues, organelles, cell types and functions > tissues types and functions > barrel cortex bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression organism description > animal > mammal > rodent > rat organism description > animal > mammal > rodent > rat |
| CSHL Authors: | |
| Communities: | CSHL labs > Yin lab |
| Depositing User: | Matt Covey |
| Date: | March 2000 |
| Date Deposited: | 28 Jan 2014 21:30 |
| Last Modified: | 28 Jan 2014 21:30 |
| PMCID: | PMC311323 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29449 |
Actions (login required)
 |
Administrator's edit/view item |