Nussaume, L., Harrison, K., Klimyuk, V., Martienssen, R., Sundaresan, V., Jones, J. D. G.
(November 1995)
Analysis of splice donor and acceptor site function in a transposable gene trap derived from the maize element Activator.
Molecular & General Genetics, 249 (1).
pp. 91-101.
ISSN 0026-8925
Abstract
Gene trap vectors have been used in insertional mutagenesis in animal systems to clone genes with interesting patterns of expression. These vectors are designed to allow the expression of a reporter gene when the vector inserts into a transcribed region. In this paper we examine alternative splicing events that result in the expression of a GUS reporter gene carried on a Ds element which has been designed as a gene trap vector for plants. We have developed a rapid and reliable method based on PCR to study such events. Many splice donor sites were observed in the 3' Ac border. The relative frequency of utilisation of certain splice donor and acceptor sites differed between tobacco and Arabidopsis. A higher stringency of splicing was observed in Arabidopsis.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
splicing
transposon tagging
intron
ac/ds
developmentally regulated genes
camv 35s-enhancer subdomains
tissue-specific expression
pre-messenger rnas
arabidopsis-thaliana
beta-glucuronidase
insitu detection
dicot plants
transposition
ac |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > introns
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons > transposon tagging
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Martienssen lab |
| Depositing User: |
Matt Covey
|
| Date: |
November 1995 |
| Date Deposited: |
12 Dec 2013 20:21 |
| Last Modified: |
12 Dec 2013 20:21 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/28997 |
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