Springer, P. S., McCombie, W. R., Sundaresan, V., Martienssen, R. A. (May 1995) Gene trap tagging of PROLIFERA, an essential MCM2-3-5-like gene in Arabidopsis. Science, 268 (5212). pp. 877-880. ISSN 0036-8075
Abstract
Gene trap transposon mutagenesis can identify essential genes whose functions in later development are obscured by an early lethal phenotype. In higher plants, many genes are required for haploid gametophyte viability, so that the phenotypic effects of their disruption cannot be readily observed in the diploid plant body. The PROLIFERA (PRL) gene, identified by gene trap transposon mutagenesis in Arabidopsis, is required for megagametophyte and embryo development. Reporter gene expression patterns revealed that PRL was expressed in dividing cells throughout the plant. PRL is related to the MCM2-3-5 family of yeast genes that are required for the initiation of DNA replication.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | mediated enhancer detection regulatory elements cell-cycle drosophila thaliana expression amplification meristem mutation excision |
| Subjects: | organism description > plant > Arabidopsis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons |
| CSHL Authors: | |
| Communities: | CSHL labs > Martienssen lab CSHL labs > McCombie lab |
| Depositing User: | Matt Covey |
| Date: | May 1995 |
| Date Deposited: | 25 Apr 2013 15:51 |
| Last Modified: | 25 Apr 2013 15:51 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28214 |
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