Hemish, J., Nakaya, N., Mittal, V., Enikolopov, G.
(October 2003)
Nitric oxide activates diverse signaling pathways to regulate gene expression.
Journal of Biological Chemistry, 278 (43).
pp. 42321-42329.
ISSN 0021-9258
Abstract
Nitric oxide signaling is crucial for effecting long lasting changes in cells, including gene expression, cell cycle arrest, apoptosis, and differentiation. We have determined the temporal order of gene activation induced by NO in mammalian cells and have examined the signaling pathways that mediate the action of NO. Using microarrays to study the kinetics of gene activation by NO, we have determined that NO induces three distinct waves of gene activity. The first wave is induced within 30 min of exposure to NO and represents the primary gene targets of NO. It is followed by subsequent waves of gene activity that may reflect further cascades of NO-induced gene expression. We verified our results using quantitative real time PCR and further validated our conclusions about the effects of NO by using cytokines to induce endogenous NO production. We next applied pharmacological and genetic approaches to determine the signaling pathways that are used by NO to regulate gene expression. We used inhibitors of particular signaling pathways, as well as cells from animals with a deleted p53 gene, to define groups of genes that require phosphatidylinositol 3-kinase, protein kinase C, NF-kappaB, p53, or combinations thereof for activation by NO. Our results demonstrate that NO utilizes several independent signaling pathways to induce gene expression.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
NF-KAPPA-B
ENDOTHELIAL-CELLS
INDUCED APOPTOSIS
P53
PROLIFERATION
FIBROBLASTS
INHIBITION
MICROARRAY
RESPONSES
ALPHA |
| Subjects: |
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > endothelial progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > endothelial progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > endothelial progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell > endothelial progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell > endothelial progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell > endothelial progenitor cell
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types
bioinformatics > genomics and proteomics > small molecules > nitric oxide
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p53
bioinformatics > genomics and proteomics > small molecules |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Enikopolov lab
CSHL labs > Mittal lab |
| Depositing User: |
Matt Covey
|
| Date: |
October 2003 |
| Date Deposited: |
22 May 2013 15:16 |
| Last Modified: |
22 May 2013 15:16 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/27972 |
Actions (login required)
 |
Administrator's edit/view item |
