Van Stry, M., Oguin Iii, T. H., Cheloufi, S., Vogel, P., Watanabe, M., Pillai, M. R., Dash, P., Thomas, P. G., Hannon, G. J., Bix, M.
(2012)
Enhanced susceptibility of Ago1/3 double-null mice to influenza a virus infection.
Journal of Virology, 86 (8).
pp. 4151-4157.
ISSN 0022538X (ISSN)
Abstract
AbstracrtRNA interference (RNAi) is a critical component of many cellular antiviral responses in plants, invertebrates, and mammals. However, its in vivo role in host protection from the negative-sense RNA virus influenza virus type A (flu) is unclear. Here we have examined the role of RNAi in host defense to flu by analyzing Argonaute 1 and 3 double-knockout mice deficient in components of the RNA-induced silencing complex. Compared to littermate controls, flu-infected double-knockout mice exhibited increased mortality, consistent with more severe alveolitis and pneumonitis. These data indicate that optimal resistance to flu requires Argonaute 1 and/or 3. Enhanced mortality of double-knockout mice was not associated either with increased viral replication or with differential pulmonary recruitment or function of innate and adaptive immune cells. Given the absence of detectable immune defects, our results support the notion that the enhanced flu susceptibility of double-knockout mice arises from an intrinsic impairment in the ability of lung cells to tolerate flu-elicited inflammation. © 2012, American Society for Microbiology.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
argonaute 1 protein
argonaute 3 protein
argonaute protein
unclassified drug
animal cell
animal experiment
animal model
animal tissue
article
controlled study
host resistance
infection sensitivity
influenza A
knockout mouse
lung alveolitis
mouse
nonhuman
pneumonia
priority journal
protein function
RNA interference
virus replication
Animals
Argonaute Proteins
Eukaryotic Initiation Factors
Genetic Predisposition to Disease
Humans
Influenza A virus
Lung
Mice
Mice, 129 Strain
Mice, Inbred C57BL
Mice, Knockout
Orthomyxoviridae Infections |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > AGO
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types
organism description > animal > mammal > rodent > mouse
organism description > virus |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hannon lab
CSHL Cancer Center Shared Resources > Animal Services
CSHL Cancer Center Shared Resources > Bioinformatics Service
CSHL Cancer Center Shared Resources > DNA Sequencing Service
CSHL Cancer Center Shared Resources > Flow Cytometry Service
CSHL Cancer Center Shared Resources > Gene Targeting Service
CSHL Cancer Center Shared Resources > Microscopy Service |
| Depositing User: |
Matt Covey
|
| Date: |
2012 |
| Date Deposited: |
09 Jan 2013 15:55 |
| Last Modified: |
26 Dec 2014 18:54 |
| PMCID: |
PMC3318639 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/26411 |
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