Vaughn, D. E., Rodriguez, J., Lazebnik, Y., Joshua-Tor, L. (1999) Crystal structure of Apaf-1 caspase recruitment domain: an alpha-helical Greek key fold for apoptotic signaling. Journal of Molecular Biology, 293 (3). pp. 439-447. ISSN 0022-2836 (Public Dataset)
Abstract
The caspase recruitment domain (CARD) of Apaf-1 binds to the CARD of caspase-9 to trigger a proteolytic cascade that leads to apoptotic cell death. We report the crystal structure of the Apaf-1 CARD at 1. 3 A resolution, solved in a two-element multiwavelength anomalous dispersion (MAD) X-ray diffraction experiment. This CARD adopts a six-helix bundle fold with Greek key topology surrounding an extensive hydrophobic core. This fold, which we call the "death fold", is found in other domains that mediate interactions in apoptotic signaling despite very low sequence identity. From a structure-based alignment, we identify conserved patterns that characterize the death fold and its subclasses. Like the Ig-fold, it provides a rigid structural scaffold upon which diverse recognition surfaces are assembled.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Motifs Amino Acid Sequence Apoptosis Apoptotic Protease-Activating Factor 1 Binding Sites Caspase 9 Caspases Conserved Sequence Crystallization Crystallography, X-Ray Humans Hydrogen Bonding Immunoglobulins Models, Molecular Molecular Sequence Data Protein Folding Protein Structure, Secondary Proteins Recombinant Fusion Proteins Signal Transduction |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis organs, tissues, organelles, cell types and functions > cell types and functions > cell functions bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein structure rendering |
| CSHL Authors: | |
| Communities: | CSHL labs > Joshua-Tor lab CSHL labs > Labeznik lab |
| Depositing User: | Matt Covey |
| Date: | 1999 |
| Date Deposited: | 12 Dec 2012 17:30 |
| Last Modified: | 06 Sep 2017 14:13 |
| Related URLs: | |
| Dataset ID: | |
| URI: | https://repository.cshl.edu/id/eprint/26393 |
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