Fasano, O., Birnbaum, D., Edlund, L., Fogh, J., Wigler, M. H. (September 1984) New human transforming genes detected by a tumorigenicity assay. Molecular & Cellular Biology, 4 (9). pp. 1695-1705.
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Abstract
A sensitive bioassay for transforming genes based on the tumorigenicity of cotransfected NIH3T3 cells in nude mice was developed. The assay differs substantially from the NIH3T3 focus assay. Using it, the transfer of 3 transforming genes from the DNA of MCF-7, a human mammary carcinoma cell line was detected. One of these is N-ras, which is amplified in MCF-7 DNA. The other two, which were called mcf2 and mcf3, do not appear to be related to known oncogenes. Their transfer could not be detected by using the NIH3T3 focus assay. Whether either mcf2 or mcf3 is associated with genetic abnormalities in MCF-7 cells is not yet known.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment > assays organs, tissues, organelles, cell types and functions > organs types and functions > breast bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | September 1984 |
| Date Deposited: | 13 Apr 2012 20:50 |
| Last Modified: | 04 Nov 2016 20:29 |
| PMCID: | PMC368975 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26177 |
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