Mutational mapping of RAS-responsive domains of the Saccharomyces cerevisiae adenylyl cyclase

Colicelli, J., Field, J., Ballester, R., Chester, N., Young, D., Wigler, M. H. (June 1990) Mutational mapping of RAS-responsive domains of the Saccharomyces cerevisiae adenylyl cyclase. Molecular & Cellular Biology, 10 (6). pp. 2539-43. ISSN 0270-7306

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URL: https://www.ncbi.nlm.nih.gov/pubmed/2111437

DOI: 10.1128/MCB.10.6.2539

Abstract

Large deletion and small insertion mutations in the adenylyl cyclase gene of Saccharomyces cerevisiae were used to map regions required for activation by RAS protein in vitro. The amino-terminal 605 amino acids were found to be dispensable for responsiveness to RAS protein. All other deletions in adenylyl cyclase destroyed its ability to respond to RAS. Small insertion mutations within the leucine-rich repeat region also prevented RAS responsiveness, while other insertions did not.

Item Type:	Paper
Uncontrolled Keywords:	Adenylate Cyclase genetics isolation & purification Cloning, Molecular DNA Fungal genetics isolation & purification Escherichia coli genetics Fungal Proteins genetics GTP-Binding Proteins genetics Genes Structural Fungal Molecular Weight Mutation Plasmids Restriction Mapping Saccharomyces cerevisiae enzymology genetics ras Proteins
Subjects:	bioinformatics > genomics and proteomics > annotation > map annotation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:	Wigler, Michael H.
Communities:	CSHL labs > Wigler lab
Depositing User:	CSHL Librarian
Date:	June 1990
Date Deposited:	19 Apr 2012 14:27
Last Modified:	04 Nov 2016 19:45
PMCID:	PMC360611
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26172

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