Muerdter, F., Olovnikov, I., Molaro, A., Rozhkov, N. V., Czech, B., Gordon, A., Hannon, G. J., Aravin, A. A. (January 2012) Production of artificial piRNAs in flies and mice. RNA, 18 (1). pp. 42-52. ISSN 1355-8382
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Abstract
In animals a discrete class of small RNAs, the piwi-interacting RNAs (piRNAs), guard germ cell genomes against the activity of mobile genetic elements. piRNAs are generated, via an unknown mechanism, from apparently single-stranded precursors that arise from discrete genomic loci, termed piRNA clusters. Presently, little is known about the signals that distinguish a locus as a source of piRNAs. It is also unknown how individual piRNAs are selected from long precursor transcripts. To address these questions, we inserted new artificial sequence information into piRNA clusters and introduced these marked clusters as transgenes into heterologous genomic positions in mice and flies. Profiling of piRNA from transgenic animals demonstrated that artificial sequences were incorporated into the piRNA repertoire. Transgenic piRNA clusters are functional in non-native genomic contexts in both mice and flies, indicating that the signals that define piRNA generative loci must lie within the clusters themselves rather than being implicit in their genomic position. Comparison of transgenic animals that carry insertions of the same artificial sequence into different ectopic piRNA-generating loci showed that both local and long-range sequence environments inform the generation of individual piRNAs from precursor transcripts.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | piwi noncoding RNA piRNA selfish genetic elements drosophila melanogaster small rnas p-elements hybrid dysgenesis dna methylation family members piwi proteins traffic jam pathway |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal |
| CSHL Authors: | |
| Communities: | CSHL Post Doctoral Fellows CSHL labs > Hannon lab CSHL Cancer Center Shared Resources > Bioinformatics Service CSHL Cancer Center Shared Resources > DNA Sequencing Service |
| Depositing User: | Editor Margaret Fantz |
| Date: | January 2012 |
| Date Deposited: | 10 Apr 2012 18:34 |
| Last Modified: | 09 Nov 2017 21:23 |
| PMCID: | PMC3261743 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26128 |
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