Human-tumor-derived cell lines contain common and different transforming genes

Perucho, M., Goldfarb, M., Shimizu, K., Lama, C., Fogh, J., Wigler, M. H. (December 1981) Human-tumor-derived cell lines contain common and different transforming genes. Cell, 27 (3 Pt 2). pp. 467-76. ISSN 0092-8674

URL: https://www.ncbi.nlm.nih.gov/pubmed/6101201

DOI: 10.1016/0092-8674(81)90388-3

Abstract

We have screened different cultured cell lines established from human tumors for the ability of their DNAs to induce transformed foci in NIH/3T3 cells. Based on restriction endonuclease digestions and the presence of human sequences in mouse transformants, we conclude that five of these human tumor cell lines contain a gene or genes capable of transforming mouse cells and that at least three different transforming genes are present in these five lines. Three cell lines, two derived from lung carcinomas and one derived from a colon carcinoma, transfer the same or closely related human genes. If these transforming genes are mediating the tumorigenic state of the human cells, then our results indicate that overlapping pathways leading to tumorigenesis may arise independently.

Item Type:	Paper
Uncontrolled Keywords:	3T3 Cells Animals Cell Transformation Neoplastic genetics DNA Neoplasm genetics Humans Mice Oncogenes Repetitive Sequences Nucleic Acid Tumor Cells Cultured metabolism
Subjects:	diseases & disorders > cancer diseases & disorders > neoplasms organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:	Wigler, Michael H.
Communities:	CSHL labs > Wigler lab
Depositing User:	CSHL Librarian
Date:	December 1981
Date Deposited:	05 Apr 2012 15:49
Last Modified:	18 Nov 2016 16:47
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26028

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