White, M. A., Nicolette, C., Minden, A., Polverino, A., Van Aelst, L., Karin, M., Wigler, M. H.
(February 1995)
Multiple Ras functions can contribute to mammalian cell transformation.
Cell, 80 (4).
pp. 533-41.
ISSN 0092-8674 (Print)
Abstract
We have developed a generalized approach, using two hybrid interactions, to isolate Ha-Ras effector loop mutations that separate the ability of Ha-Ras to interact with different downstream effectors. These mutations attenuate or eliminate Ha-ras(G12V) transformation of mammalian cells, but retain complementary activity, as demonstrated by synergistic induction of foci of growth-transformed cells, and by the ability to activate different downstream components. The transformation defect of Ha-ras(G12V, E37G) is rescued by a mutant, raf1, that restores interaction. These results indicate that multiple cellular components, including Raf1, are activated by Ha-Ras and contribute to Ha-Ras-induced mammalian cell transformation.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Animals
Cell Transformation Neoplastic genetics
Cells Cultured
Chloramphenicol O-Acetyltransferase analysis
Gene Library
Genes, ras genetics
Genetic Complementation Test
Humans
Mice
Mutation
Protein Kinases analysis
Protein Serine Threonine Kinases genetics
Proto-Oncogene Proteins genetics
Proto-Oncogene Proteins c-raf
Rats
Recombinant Fusion Proteins biosynthesis
Saccharomyces cerevisiae genetics
Schizosaccharomyces genetics
Transfection |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Van Aelst lab
CSHL labs > Wigler lab
CSHL labs > Stillman lab |
| Depositing User: |
CSHL Librarian
|
| Date: |
24 February 1995 |
| Date Deposited: |
06 Apr 2012 14:55 |
| Last Modified: |
18 Nov 2016 17:08 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/25981 |
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