Wigler, M. H., Levy, D., Perucho, M. (1981) The somatic replication of DNA methylation. Cell, 24 (1). pp. 33-40.
Abstract
The hypothesis that DNA methylation patterns are replicated in the somatic cells of vertebrates was tested. M-Hpa II, the modification enzyme from Hemophilus parainfluenzae, was used to methylate bacteriophage .vphi.X174 RF DNA and the cloned chicken thymidine kinase (tk) gene in vitro and then to introduce these DNA and unmethylated controls into tk- cultured mouse cells by DNA-mediated transformation. Twenty-five cell generations later, the state of methylation of transferred DNA was examined by restriction endonuclease analysis and blot hybridization. Methylation at Hpa II sites is replicated by these cultured cells but not with 100% fidelity. Methylation of the cloned chicken tk gene decreases its apparent transformation efficiency relative to unmethylated molecules.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | 1981 |
| Date Deposited: | 06 Apr 2012 15:56 |
| Last Modified: | 18 Nov 2016 17:00 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25966 |
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