Wigler, M. H., Pellicer, A., Silverstein, S., Axel, R., Urlaub, G., Chasin, L. (March 1979) DNA-Mediated Transfer of the Adenine Phosphoribosyltransferase Locus into Mammalian Cells. Proc Natl Acad Sci U S A, 76 (3). pp. 1373-1376.
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Abstract
In this report, we demonstrate the feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase (aprt; AMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.7) to the aprt+ phenotype by means of DNA-mediated gene transfer. Transformation was effected by using unfractionated high molecular weight genomic DNA from Chinese hamster, human, and mouse cells and restriction endonuclease-digested DNA from rabbit liver. The transformation frequency observed was between 1 and 10 colonies per 106 cells per 20 {micro} g of donor DNA. Transformants displayed enzymatic activity that was donor derived as demonstrated by isoelectric focusing of cytoplasmic extracts. These transformants fall into two classes: those that are phenotypically stable when grown in the absence of selective pressure and those that are phenotypically unstable under the same conditions.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene transfer |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | 1 March 1979 |
| Date Deposited: | 06 Apr 2012 15:47 |
| Last Modified: | 09 Nov 2017 21:00 |
| PMCID: | PMC383253 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25963 |
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