Irvine, D. V., Zaratiegui, Mi., Tolia, N. H., Goto, D. B., Chitwood, D. H., Vaughn, M. W., Joshua-Tor, L., Martienssen, R. A.
(August 2006)
Argonaute slicing is required for heterochromatic silencing and spreading.
Science, 313 (5790).
pp. 1134-7.
ISSN 1095-9203 (Electronic)
Abstract
Small interfering RNA (siRNA) guides dimethylation of histone H3 lysine-9 (H3K9me2) via the Argonaute and RNA-dependent RNA polymerase complexes, as well as base-pairing with either RNA or DNA. We show that Argonaute requires the conserved aspartate-aspartate-histidine motif for heterochromatic silencing and for ribonuclease H-like cleavage (slicing) of target messages complementary to siRNA. In the fission yeast Schizosaccharomyces pombe, heterochromatic repeats are transcribed by polymerase II. We show that H3K9me2 spreads into silent reporter genes when they are embedded within these transcripts and that spreading requires read-through transcription, as well as slicing by Argonaute. Thus, siRNA guides histone modification by basepairing interactions with RNA.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Amino Acid Motifs
Base Pairing
Genes
Reporter
Heterochromatin
genetics metabolism
Histones
metabolism
RNA Interference
RNA Fungal
metabolism
RNA Messenger
metabolism
RNA Small Interfering metabolism
Recombinant Fusion Proteins/metabolism
Schizosaccharomyces genetics/metabolism
Schizosaccharomyces pombe Proteins
chemistry
genetics/ metabolism
Transcription Genetic |
| Subjects: |
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Joshua-Tor lab
CSHL labs > Martienssen lab
School of Biological Sciences > Publications |
| Depositing User: |
CSHL Librarian
|
| Date: |
25 August 2006 |
| Date Deposited: |
14 Dec 2011 15:35 |
| Last Modified: |
03 Oct 2019 13:50 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/22827 |
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