Boivin, B., Tonks, N. K. , Yang, M. (August 2010) Targeting the reversibly oxidized protein tyrosine phosphatase superfamily. Science Signaling, 3 (137).
Abstract
Controlled production of reactive oxygen species leads to reversible oxidation of protein tyrosine phosphatases (PTPs) and has emerged as an important tier of regulation over phosphorylation-dependent signal transduction. We present a modified cysteinyl-labeling assay that detects reversible oxidation of members of each of the different PTP subclasses. Here, we describe the methods for enriching reversibly oxidized PTPs from complex protein extracts, illustrating the procedure in IMR90 fibroblasts.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > design > protein design bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > Mapping and Rendering > Structure Rendering bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > tyrosine kinase |
| CSHL Authors: | |
| Communities: | CSHL labs > Tonks lab |
| Depositing User: | CSHL Librarian |
| Date: | 31 August 2010 |
| Date Deposited: | 27 Sep 2011 14:52 |
| Last Modified: | 21 Feb 2017 20:03 |
| PMCID: | PMC3418810 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/15364 |
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