Era of gapless plant genomes: innovations in sequencing and mapping technologies revolutionize genomics and breeding

Gladman, Nicholas, Goodwin, Sara, Chougule, Kapeel, Richard McCombie, William, Ware, Doreen (January 2023) Era of gapless plant genomes: innovations in sequencing and mapping technologies revolutionize genomics and breeding. Current Opinion in Biotechnology, 79. p. 102886. ISSN 0958-1669

PDF 2023-Ware-Era-of-gapless-plant-genomes-innovations-in-sequencing-and-mapping-technologies-revolutionize-genomics-and-breeding.pdf Available under License Creative Commons Attribution. Download (1MB)

Abstract

Whole-genome sequencing and assembly have revolutionized plant genetics and molecular biology over the last two decades. However, significant shortcomings in first- and second-generation technology resulted in imperfect reference genomes: numerous and large gaps of low quality or undeterminable sequence in areas of highly repetitive DNA along with limited chromosomal phasing restricted the ability of researchers to characterize regulatory noncoding elements and genic regions that underwent recent duplication events. Recently, advances in long-read sequencing have resulted in the first gapless, telomere-to-telomere (T2T) assemblies of plant genomes. This leap forward has the potential to increase the speed and confidence of genomics and molecular experimentation while reducing costs for the research community.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics Investigative techniques and equipment Investigative techniques and equipment > assays bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > telomeres Investigative techniques and equipment > assays > whole genome sequencing
CSHL Authors:	Gladman, Nicholas Goodwin, Sara Chougule, Kapeel Ware, Doreen H. McCombie, W. Richard
Communities:	CSHL labs > McCombie lab CSHL labs > Ware lab CSHL Cancer Center Program CSHL Cancer Center Program > Cancer Genetics and Genomics Program
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	12 January 2023
Date Deposited:	23 Jan 2023 19:47
Last Modified:	09 Feb 2024 14:36
PMCID:	PMC9899316
URI:	https://repository.cshl.edu/id/eprint/40798

Actions (login required)

Administrator's edit/view item