Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases.

Taranda, Julian, Mathew, Grinu, Watrud, Kaitlin, El-Amine, Nour, Lee, Matthew F, Elowsky, Corey, Bludova, Anastasiia, Escobar Avelar, Sintia, Nowak, Dawid G, Wee, Tse-Luen, Wilkinson, John E, Trotman, Lloyd C, Osten, Pavel (November 2021) Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases. Cell Reports, 37 (7). p. 110027. ISSN 2211-1247

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Abstract

Early steps of cancer initiation and metastasis, while critical for understanding disease mechanisms, are difficult to visualize and study. Here, we describe an approach to study the processes of initiation, progression, and metastasis of prostate cancer (PC) in a genetically engineered RapidCaP mouse model, which combines whole-organ imaging by serial two-photon tomography (STPT) and post hoc thick-section immunofluorescent (IF) analysis. STPT enables the detection of single tumor-initiating cells within the entire prostate, and consequent IF analysis reveals a transition from normal to transformed epithelial tissue and cell escape from the tumor focus. STPT imaging of the liver and brain reveal the distribution of multiple metastatic foci in the liver and an early-stage metastatic cell invasion in the brain. This imaging and data analysis pipeline can be readily applied to other mouse models of cancer, offering a highly versatile whole-organ platform to study in situ mechanisms of cancer initiation and progression.

Item Type:	Paper
Subjects:	Investigative techniques and equipment > imaging diseases & disorders > cancer > metastasis diseases & disorders > cancer > cancer types > prostate cancer
CSHL Authors:	Taranda, Julian Mathew, Grinu El-Amine, Nour Lee, Matthew Elowsky, Corey Nowak, Dawid G. Trotman, Lloyd C. Osten, Pavel Bludova, Anastasiia Escobar Avelar, Sintia Wee, Tse-Luen Watrud, Kaitlin
Communities:	CSHL labs > Mitra lab CSHL labs > Osten lab CSHL labs > Spector lab CSHL labs > Trotman lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	16 November 2021
Date Deposited:	06 Dec 2021 15:29
Last Modified:	06 Dec 2021 15:29
URI:	https://repository.cshl.edu/id/eprint/40434

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