Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases

Taranda, Julian, Mathew, Grinu, Watrud, Kaitlin, El-Amine, Nour, Lee, Matthew F, Elowsky, Corey, Bludova, Anastasiia, Escobar Avelar, Sintia, Nowak, Dawid G, Wee, Tse-Luen, Wilkinson, John E, Trotman, Lloyd C, Osten, Pavel (November 2021) Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases. Cell Reports, 37 (7). p. 110027. ISSN 2211-1247

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URL: https://www.ncbi.nlm.nih.gov/pubmed/34788609

DOI: 10.1016/j.celrep.2021.110027

Abstract

Early steps of cancer initiation and metastasis, while critical for understanding disease mechanisms, are difficult to visualize and study. Here, we describe an approach to study the processes of initiation, progression, and metastasis of prostate cancer (PC) in a genetically engineered RapidCaP mouse model, which combines whole-organ imaging by serial two-photon tomography (STPT) and post hoc thick-section immunofluorescent (IF) analysis. STPT enables the detection of single tumor-initiating cells within the entire prostate, and consequent IF analysis reveals a transition from normal to transformed epithelial tissue and cell escape from the tumor focus. STPT imaging of the liver and brain reveal the distribution of multiple metastatic foci in the liver and an early-stage metastatic cell invasion in the brain. This imaging and data analysis pipeline can be readily applied to other mouse models of cancer, offering a highly versatile whole-organ platform to study in situ mechanisms of cancer initiation and progression.

Item Type:	Paper
Subjects:	diseases & disorders Investigative techniques and equipment diseases & disorders > neoplasms organism description > animal Investigative techniques and equipment > imaging organism description > animal > mammal diseases & disorders > cancer > metastasis organism description > animal > mammal > rodent > mouse diseases & disorders > cancer > cancer types > prostate cancer organism description > animal > mammal > rodent
CSHL Authors:	Taranda, Julian Mathew, Grinu El-Amine, Nour Lee, Matthew Elowsky, Corey Nowak, Dawid G. Trotman, Lloyd C. Osten, Pavel Bludova, Anastasiia Escobar Avelar, Sintia Wee, Tse-Luen Watrud, Kaitlin
Communities:	CSHL Cancer Center Program CSHL Cancer Center Program > Cellular Communication in Cancer Program CSHL Cancer Center Shared Resources > Microscopy Service CSHL labs > Mitra lab CSHL labs > Osten lab CSHL labs > Spector lab CSHL labs > Trotman lab School of Biological Sciences > Publications
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	16 November 2021
Date Deposited:	06 Dec 2021 15:29
Last Modified:	29 Feb 2024 19:35
PMCID:	PMC9159673
URI:	https://repository.cshl.edu/id/eprint/40434

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