A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy-induced Cancer Cell Death

Barth, Nicole, Mendive-Tapia, Lorena, Subiros-Funosas, Ramon, Ghashghaie, Ouldouz, Lavilla, Rodolfo, Maiorino, Laura, He, Xue-Yan, Dransfield, Ian, Egeblad, Mikala, Vendrell, Marc (November 2021) A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy-induced Cancer Cell Death. Angewandte Chemie International Edition. ISSN 1433-7851

URL: https://www.ncbi.nlm.nih.gov/pubmed/34762762
DOI: 10.1002/anie.202113020

Abstract

The detection and quantification of apoptotic cells is a key process in cancer research, particularly during the screening of anticancer therapeutics and in mechanistic studies using preclinical models. Intravital optical imaging enables high-resolution visualisation of cellular events in live organisms; however, there are few fluorescent probes that can reliably provide functional readouts in situ without interference from tissue autofluorescence. Here we report the design and optimisation of the fluorogenic probe Apotracker Red for real-time detection of cancer cell death. The strong fluorogenic behaviour, high selectivity, and excellent stability of Apotracker Red make it a reliable optical reporter for the characterisation of the effects of anticancer drugs in cells in vitro and for direct imaging of chemotherapy-induced apoptosis in vivo in mouse models of breast cancer.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
diseases & disorders > cancer > cancer types > breast cancer
Investigative techniques and equipment > imaging > intravital imaging
CSHL Authors:
Communities: CSHL labs > Egeblad lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 11 November 2021
Date Deposited: 17 Nov 2021 16:24
Last Modified: 11 Apr 2022 13:47
PMCID: PMC8991960
URI: https://repository.cshl.edu/id/eprint/40424

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