Barth, Nicole, Mendive-Tapia, Lorena, Subiros-Funosas, Ramon, Ghashghaie, Ouldouz, Lavilla, Rodolfo, Maiorino, Laura, He, Xue-Yan, Dransfield, Ian, Egeblad, Mikala, Vendrell, Marc (November 2021) A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy-induced Cancer Cell Death. Angewandte Chemie International Edition. ISSN 1433-7851
Abstract
The detection and quantification of apoptotic cells is a key process in cancer research, particularly during the screening of anticancer therapeutics and in mechanistic studies using preclinical models. Intravital optical imaging enables high-resolution visualisation of cellular events in live organisms; however, there are few fluorescent probes that can reliably provide functional readouts in situ without interference from tissue autofluorescence. Here we report the design and optimisation of the fluorogenic probe Apotracker Red for real-time detection of cancer cell death. The strong fluorogenic behaviour, high selectivity, and excellent stability of Apotracker Red make it a reliable optical reporter for the characterisation of the effects of anticancer drugs in cells in vitro and for direct imaging of chemotherapy-induced apoptosis in vivo in mouse models of breast cancer.
| Item Type: | Paper |
|---|---|
| Subjects: | diseases & disorders > cancer diseases & disorders organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis diseases & disorders > cancer > cancer types > breast cancer organs, tissues, organelles, cell types and functions > cell types and functions > cell functions organs, tissues, organelles, cell types and functions > cell types and functions Investigative techniques and equipment > imaging > intravital imaging organs, tissues, organelles, cell types and functions diseases & disorders > cancer > cancer types |
| CSHL Authors: | |
| Communities: | CSHL Cancer Center Program > Cellular Communication in Cancer Program CSHL labs > Egeblad lab School of Biological Sciences > Publications |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 11 November 2021 |
| Date Deposited: | 17 Nov 2021 16:24 |
| Last Modified: | 29 Feb 2024 21:01 |
| PMCID: | PMC8991960 |
| URI: | https://repository.cshl.edu/id/eprint/40424 |
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