Enhancing grain-yield-related traits by CRISPR-Cas9 promoter editing of maize CLE genes.

Liu, Lei, Gallagher, Joseph, Arevalo, Edgar Demesa, Chen, Richelle, Skopelitis, Tara, Wu, Qingyu, Bartlett, Madelaine, Jackson, David (March 2021) Enhancing grain-yield-related traits by CRISPR-Cas9 promoter editing of maize CLE genes. Nature Plants, 7 (3). pp. 287-294. ISSN 2055-026X

URL: https://www.ncbi.nlm.nih.gov/pubmed/33619356
DOI: 10.1038/s41477-021-00858-5

Abstract

Several yield-related traits selected during crop domestication and improvement1,2 are associated with increases in meristem size3, which is controlled by CLE peptide signals in the CLAVATA-WUSCHEL pathway4-13. Here, we engineered quantitative variation for yield-related traits in maize by making weak promoter alleles of CLE genes, and a null allele of a newly identified partially redundant compensating CLE gene, using CRISPR-Cas9 genome editing. These strategies increased multiple maize grain-yield-related traits, supporting the enormous potential for genomic editing in crop enhancement.

Item Type: Paper
Subjects: organism description > plant > maize
Investigative techniques and equipment > CRISPR-Cas9
organs, tissues, organelles, cell types and functions > tissues types and functions > meristem
CSHL Authors:
Communities: CSHL labs > Jackson lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: March 2021
Date Deposited: 30 Apr 2021 20:29
Last Modified: 01 Jun 2021 15:30
URI: https://repository.cshl.edu/id/eprint/39967

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