Liu, Lei, Gallagher, Joseph, Arevalo, Edgar Demesa, Chen, Richelle, Skopelitis, Tara, Wu, Qingyu, Bartlett, Madelaine, Jackson, David
(March 2021)
Enhancing grain-yield-related traits by CRISPR-Cas9 promoter editing of maize CLE genes.
Nature Plants, 7 (3).
pp. 287-294.
ISSN 2055-026X
Abstract
Several yield-related traits selected during crop domestication and improvement1,2 are associated with increases in meristem size3, which is controlled by CLE peptide signals in the CLAVATA-WUSCHEL pathway4-13. Here, we engineered quantitative variation for yield-related traits in maize by making weak promoter alleles of CLE genes, and a null allele of a newly identified partially redundant compensating CLE gene, using CRISPR-Cas9 genome editing. These strategies increased multiple maize grain-yield-related traits, supporting the enormous potential for genomic editing in crop enhancement.
| Item Type: |
Paper
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| Subjects: |
bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
Investigative techniques and equipment
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
Investigative techniques and equipment > CRISPR-Cas9
organs, tissues, organelles, cell types and functions > tissues types and functions > meristem
organs, tissues, organelles, cell types and functions
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > plant proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > tissues types and functions > signal transduction
organs, tissues, organelles, cell types and functions > tissues types and functions |
| CSHL Authors: |
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| Communities: |
CSHL labs > Jackson lab |
| SWORD Depositor: |
CSHL Elements
|
| Depositing User: |
CSHL Elements
|
| Date: |
March 2021 |
| Date Deposited: |
30 Apr 2021 20:29 |
| Last Modified: |
25 Jan 2024 15:03 |
| URI: |
https://repository.cshl.edu/id/eprint/39967 |
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