HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics

Marchione, D. M., Ilieva, I., Devins, K., Sharpe, D., Pappin, D. J., Garcia, B. A., Wilson, J. P., Wojcik, J. B. (January 2020) HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics. J Proteome Res. ISSN 1535-3893 (Public Dataset)

URL: https://www.ncbi.nlm.nih.gov/pubmed/31935107
DOI: 10.1021/acs.jproteome.9b00686

Abstract

Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing an invaluable resource for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present high-yield protein extraction and recovery by direct solubilization (HYPERsol), an optimized workflow using ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samples comparable to that achieved from flash-frozen tissue (average R = 0.936). When applied to archival samples, HYPERsol resulted in high-quality data from FFPE specimens in storage for up to 17 years, and may enable the discovery of new immunohistochemical markers.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics
Investigative techniques and equipment > staining techniques
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Adrian Gomez
Date: 14 January 2020
Date Deposited: 16 Jan 2020 16:10
Last Modified: 16 Jan 2020 16:10
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Dataset ID:
  • Supporting Information is available at https://pubs.acs.org/doi/10.1021/acs.jproteome.9b00686.
URI: https://repository.cshl.edu/id/eprint/38907

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