Marchione, D. M., Ilieva, I., Devins, K., Sharpe, D., Pappin, D. J., Garcia, B. A., Wilson, J. P., Wojcik, J. B. (January 2020) HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics. J Proteome Res, 19 (2). pp. 973-983. ISSN 1535-3893 (Public Dataset)
Abstract
Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing an invaluable resource for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present high-yield protein extraction and recovery by direct solubilization (HYPERsol), an optimized workflow using ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samples comparable to that achieved from flash-frozen tissue (average R = 0.936). When applied to archival samples, HYPERsol resulted in high-quality data from FFPE specimens in storage for up to 17 years, and may enable the discovery of new immunohistochemical markers.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics bioinformatics > genomics and proteomics Investigative techniques and equipment > staining techniques |
| CSHL Authors: | |
| Communities: | CSHL Cancer Center Program > Cellular Communication in Cancer Program CSHL labs > Pappin lab |
| Depositing User: | Adrian Gomez |
| Date: | 14 January 2020 |
| Date Deposited: | 16 Jan 2020 16:10 |
| Last Modified: | 01 Feb 2024 16:07 |
| PMCID: | PMC7515636 |
| Related URLs: | |
| Dataset ID: |
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| URI: | https://repository.cshl.edu/id/eprint/38907 |
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