Creating an miR30-based shRNA vector

Chang, K., Marran, K., Valentine, A., Hannon, G. J. (2013) Creating an miR30-based shRNA vector. Cold Spring Harbor Protocols, 8 (7). pp. 631-635. ISSN 19403402

URL: https://www.scopus.com/inward/record.uri?eid=2-s2...
DOI: 10.1101/pdb.prot075853

Abstract

Generating expression constructs for artificial microRNAs (miRNAs) is relatively straightforward. This protocol describes the creation of miR-30-based short hairpin RNA (shRNA) cassettes that are compatible with a number of standard vector systems. The principles outlined here can also be easily applied to other miRNA scaffolds or to simple snapback shRNAs. It is important to note that one must understand the processing of the artificial scaffold and be able to predict precisely the small RNAs that will be generated. Otherwise, no design principles can be effectively applied and the probability that any individual shRNA clone will work effectively will be greatly reduced.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > non-coding RNA
CSHL Authors:
Communities: CSHL labs > Chang lab
CSHL labs > Hannon lab
Depositing User: Adrian Gomez
Date: 2013
Date Deposited: 22 Nov 2019 20:09
Last Modified: 22 Nov 2019 20:09
Related URLs:
URI: https://repository.cshl.edu/id/eprint/38724

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