Oral mucosal organoids as a potential platform for personalized cancer therapy

Driehuis, E., Kolders, S., Spelier, S., Lohmussaar, K., Willems, S. M., Devriese, L. A., de Bree, R., de Ruiter, E. J., Korving, J., Begthel, H., Van Es, J. H., Geurts, V., He, G. W., van Jaarsveld, R. H., Oka, R., Muraro, M. J., Vivie, J., Zandvliet, Mmjm, Hendrickx, A. P. A., Iakobachvili, N., Sridevi, P., Kranenburg, O., van Boxtel, R., Kops, Gjpl, Tuveson, D. A., Peters, P. J., van Oudenaarden, A., Clevers, H. (May 2019) Oral mucosal organoids as a potential platform for personalized cancer therapy. Cancer Discov, 9 (7). pp. 852-871. ISSN 2159-8274

URL: https://www.ncbi.nlm.nih.gov/pubmed/31053628
DOI: 10.1158/2159-8290.cd-18-1522

Abstract

Previous studies have described that tumor organoids can capture the diversity of defined human carcinoma types. Here, we describe conditions for long-term culture of human mucosal organoids. Using this protocol, a panel of 31 head and neck squamous cell carcinoma (HNSCC)-derived organoid lines was established. This panel recapitulates genetic and molecular characteristics previously described for HNSCC. Organoids retain their tumorigenic potential upon xenotransplantation. We observe differential responses to a panel of drugs including cisplatin, carboplatin, cetuximab and radiotherapy in vitro. Drug screens reveals selective sensitivity to targeted drugs that are not normally used in the treatment of HNSCC patients. These observations may inspire a personalized approach to the management of HNSCC and expand the repertoire of HNSCC drugs.

Item Type: Paper
Subjects: diseases & disorders > cancer
diseases & disorders
Investigative techniques and equipment
diseases & disorders > neoplasms
organism description > animal
Investigative techniques and equipment > cell culture > cancer organoids
Investigative techniques and equipment > cell culture
diseases & disorders > cancer > drugs and therapies > chemotherapy
diseases & disorders > cancer > drugs and therapies
organism description > animal > mammal
organism description > animal > mammal > rodent > mouse
therapies > personalised therapy
diseases & disorders > cancer > drugs and therapies > precision medicine
organism description > animal > mammal > rodent
CSHL Authors:
Communities: CSHL labs > Tuveson lab
CSHL Cancer Center Program > Cellular Communication in Cancer Program
Depositing User: Matthew Dunn
Date: 3 May 2019
Date Deposited: 22 May 2019 15:05
Last Modified: 02 Feb 2024 14:49
Related URLs:
URI: https://repository.cshl.edu/id/eprint/37826

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