Expression of knotted1 marks shoot meristem formation during maize embryogenesis

Smith, Laurie G., Jackson, David, Hake, Sarah (1995) Expression of knotted1 marks shoot meristem formation during maize embryogenesis. Developmental Genetics, 16 (4). pp. 344-348. ISSN 1520-6408

DOI: 10.1002/dvg.1020160407


The formation of shoot and root meristems that ultimately give rise to all tissues of the plant body occurs for the first time during embryogenesis. Meristem formation has traditionally been defined in terms of the appearance of histological features of meristems; this approach has led to varying interpretations of the timing of meristem formation relative to other events in embryogenesis. Markers that would provide more objective criteria for the analysis of meristem formation have not been widely available. The maize homeobox gene, knotted1 (kn1), is expressed in shoot meristems throughout postembryonic stages of shoot development. In order to determine whether this gene is expressed in the shoot meristem from its earliest inception, we examined the expression of kn1 in embryos at a series of stages by in situ hybridization to kn1 mRNA and immunolocalization of KN1 protein. Our results show that the onset of kn1 expression is temporally and spatially coincident with the earliest histologically recognizable signs of shoot meristem formation in the embryo, and thus provides a valuable marker for this process. © 1995 Wiley-Liss, Inc.

Item Type: Paper
Uncontrolled Keywords: knotted1 embryogenesis shoot apical meristem
Subjects: organism description > plant > maize
organs, tissues, organelles, cell types and functions > tissues types and functions > apical meristem
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > homeobox genes
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: Matt Covey
Date: 1995
Date Deposited: 12 May 2014 15:36
Last Modified: 12 May 2014 15:36

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving