Ordered progression of stage-specific miRNA profiles in the mouse B2 B-cell lineage

Spierings, D. C., McGoldrick, D., Hamilton-Easton, A. M., Neale, G., Murchison, E. P., Hannon, G. J., Green, D. R., Withoff, S. (2011) Ordered progression of stage-specific miRNA profiles in the mouse B2 B-cell lineage. Blood, 117 (20). pp. 5340-5349. ISSN 00064971 (ISSN)

Abstract

Micro-RNAs (miRNAs) have been recognized as critical regulators of gene expression, and deregulation of miRNA expression has been implicated in a wide spectrumofdiseases. To provide aframework for the role of miRNAs in B-cell development and malignancy, we deepsequenced miRNAs from B1 cells and 10 developmental stages that can be identified within the mouse B2 B-cell lineage. The expression profiles of the 232 known miRNAs that are expressed during B-cell development display stage-specific induction patterns, yet hierarchical clustering analysis showed relationships that are in full agreement with the model of the B2 B-cell developmental pathway. Analysis of exemplary miRNA expression profiles (miR-150, miR-146a, miR-155, miR-181) confirmed that our data are in agreement with previous results. The high resolution of the expression data allowed for the identification of the sequential expression of oncomir-1/miR-17-92 and its paralogs miR-106a-363 and miR-106b-25 in subsequent developmental stages in the BM. Further, we have identified and validated 45 novel miRNAs and 6 novel miRNA candidates expressed in developing B cells. © 2011 by The American Society of Hematology.

Item Type:	Paper
Uncontrolled Keywords:	microRNA microRNA 106a microRNA 106b microRNA 146a microRNA 150 microRNA 155 microRNA 181 unclassified drug animal cell article B lymphocyte cell maturation deregulation developmental stage disease course gene expression mouse nonhuman priority journal protein expression Animals B-Lymphocyte Subsets Cell Differentiation Cell Lineage Cell Transformation, Neoplastic Gene Expression Profiling Gene Expression Regulation, Developmental Mice Mice, Inbred C57BL MicroRNAs RNA, Untranslated
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA organism description > animal > mammal > rodent > mouse
CSHL Authors:	Hannon, Gregory J. Murchison, Elizabeth P.
Communities:	CSHL Cancer Center Program > Cancer Genetics CSHL Cancer Center Shared Resources > Bioinformatics Service CSHL Cancer Center Shared Resources > DNA Sequencing Service CSHL Cancer Center Shared Resources > Flow Cytometry Service CSHL labs > Hannon lab School of Biological Sciences > Publications
Depositing User:	Matt Covey
Date:	2011
Date Deposited:	09 Jan 2013 16:17
Last Modified:	03 Jan 2019 21:37
PMCID:	PMC3109709
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26426

Actions (login required)

Administrator's edit/view item