Spierings, D. C., McGoldrick, D., Hamilton-Easton, A. M., Neale, G., Murchison, E. P., Hannon, G. J., Green, D. R., Withoff, S.
(2011)
Ordered progression of stage-specific miRNA profiles in the mouse B2 B-cell lineage.
Blood, 117 (20).
pp. 5340-5349.
ISSN 00064971 (ISSN)
Abstract
Micro-RNAs (miRNAs) have been recognized as critical regulators of gene expression, and deregulation of miRNA expression has been implicated in a wide spectrumofdiseases. To provide aframework for the role of miRNAs in B-cell development and malignancy, we deepsequenced miRNAs from B1 cells and 10 developmental stages that can be identified within the mouse B2 B-cell lineage. The expression profiles of the 232 known miRNAs that are expressed during B-cell development display stage-specific induction patterns, yet hierarchical clustering analysis showed relationships that are in full agreement with the model of the B2 B-cell developmental pathway. Analysis of exemplary miRNA expression profiles (miR-150, miR-146a, miR-155, miR-181) confirmed that our data are in agreement with previous results. The high resolution of the expression data allowed for the identification of the sequential expression of oncomir-1/miR-17-92 and its paralogs miR-106a-363 and miR-106b-25 in subsequent developmental stages in the BM. Further, we have identified and validated 45 novel miRNAs and 6 novel miRNA candidates expressed in developing B cells. © 2011 by The American Society of Hematology.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
microRNA
microRNA 106a
microRNA 106b
microRNA 146a
microRNA 150
microRNA 155
microRNA 181
unclassified drug
animal cell
article
B lymphocyte
cell maturation
deregulation
developmental stage
disease course
gene expression
mouse
nonhuman
priority journal
protein expression
Animals
B-Lymphocyte Subsets
Cell Differentiation
Cell Lineage
Cell Transformation, Neoplastic
Gene Expression Profiling
Gene Expression Regulation, Developmental
Mice
Mice, Inbred C57BL
MicroRNAs
RNA, Untranslated |
| Subjects: |
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
organism description > animal > mammal > rodent > mouse |
| CSHL Authors: |
|
| Communities: |
CSHL Cancer Center Program > Cancer Genetics
CSHL Cancer Center Shared Resources > Bioinformatics Service
CSHL Cancer Center Shared Resources > DNA Sequencing Service
CSHL Cancer Center Shared Resources > Flow Cytometry Service
CSHL labs > Hannon lab
School of Biological Sciences > Publications |
| Depositing User: |
Matt Covey
|
| Date: |
2011 |
| Date Deposited: |
09 Jan 2013 16:17 |
| Last Modified: |
03 Jan 2019 21:37 |
| PMCID: |
PMC3109709 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/26426 |
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