Chang, K., Marran, K., Valentine, A., Hannon, G. J. (2013) Creating an miR30-based shRNA vector. Cold Spring Harbor Protocols, 8 (7). pp. 631-635. ISSN 19403402
Abstract
Generating expression constructs for artificial microRNAs (miRNAs) is relatively straightforward. This protocol describes the creation of miR-30-based short hairpin RNA (shRNA) cassettes that are compatible with a number of standard vector systems. The principles outlined here can also be easily applied to other miRNA scaffolds or to simple snapback shRNAs. It is important to note that one must understand the processing of the artificial scaffold and be able to predict precisely the small RNAs that will be generated. Otherwise, no design principles can be effectively applied and the probability that any individual shRNA clone will work effectively will be greatly reduced.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > non-coding RNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Chang lab CSHL labs > Hannon lab |
| Depositing User: | Adrian Gomez |
| Date: | 2013 |
| Date Deposited: | 22 Nov 2019 20:09 |
| Last Modified: | 22 Nov 2019 20:09 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/38724 |
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