CRISPR-Cas9 a boon or bane: the bumpy road ahead to cancer therapeutics

Ghosh, D., Venkataramani, P., Nandi, S., Bhattacharjee, S. (January 2019) CRISPR-Cas9 a boon or bane: the bumpy road ahead to cancer therapeutics. Cancer Cell Int, 19. p. 12. ISSN 1475-2867 (Print)1475-2867

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URL: https://www.ncbi.nlm.nih.gov/pubmed/30636933
DOI: 10.1186/s12935-019-0726-0

Abstract

Genome editing allows for the precise manipulation of DNA sequences in a cell making this technology essential for understanding gene function. CRISPR/Cas9 is a targeted genome-editing platform derived from bacterial adaptive immune system and has been repurposed into a genome-editing tool. The RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, making this technology easier, more efficient, scalable and an indispensable tool in biological research. This technology has helped genetically engineer animal models to understand disease mechanisms and elucidate molecular details that can be exploited for improved therapeutic outcomes. In this review, we describe the CRISPR-Cas9 gene-editing mechanism, CRISPR-screening methods, therapeutic targeting of CRISPR in animal models and in cancer immunotherapy. We also discuss the ongoing clinical trials using this tool, limitations of this tool that might impede the clinical applicability of CRISPR-Cas9 and future directions for developing effective CRISPR-Cas9 delivery systems that may improve cancer therapeutics.

Item Type: Paper
Subjects: Investigative techniques and equipment > CRISPR-Cas9
diseases & disorders > cancer > drugs and therapies > Immunotherapy
CSHL Authors:
Communities: CSHL labs > Lee lab
CSHL labs > Martienssen lab
CSHL labs > Stillman lab
CSHL labs > Tonks lab
Depositing User: Matthew Dunn
Date: 8 January 2019
Date Deposited: 29 Jan 2019 15:43
Last Modified: 29 Jan 2019 15:43
PMCID: PMC6325665
Related URLs:
URI: http://repository.cshl.edu/id/eprint/37673

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