Detection of MAPK3/6 Phosphorylation During Hypersensitive Response (HR)-Associated Programmed Cell Death in Plants

Wu, Q., Jackson, D. (January 2018) Detection of MAPK3/6 Phosphorylation During Hypersensitive Response (HR)-Associated Programmed Cell Death in Plants. Methods Mol Biol, 1743. pp. 153-161. ISSN 1064-3745

URL: https://www.ncbi.nlm.nih.gov/pubmed/29332294
DOI: 10.1007/978-1-4939-7668-3_14

Abstract

Programmed cell death (PCD) is an essential component of development, biotic and abiotic responses. Hypersensitive response (HR)-associated cell death activated under pathogen attack is one of the most dramatic manifestations of PCD in plants. Signal transduction through mitogen-activated protein kinase (MAPK) cascades, a very conserved signaling pathway across eukaryotes, is a core mediator for HR-associated PCD. Therefore, monitoring MAPK activation enables the mechanisms underlying HR-associated PCD to be elucidated. Here, we describe the use of a phosphorylation-specific MAPK3/6 antibody to monitor the activation of MAPK3/6 during HR-associated PCD. The technique may be adapted for use in other types of PCD.

Item Type: Paper
Uncontrolled Keywords: Hypersensitive response Mapk Phosphorylation Programmed cell death
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
organism description > plant
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: Matt Covey
Date: January 2018
Date Deposited: 26 Jan 2018 17:55
Last Modified: 26 Jan 2018 17:55
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35982

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