Wu, Q., Jackson, D. (January 2018) Detection of MAPK3/6 Phosphorylation During Hypersensitive Response (HR)-Associated Programmed Cell Death in Plants. Methods Mol Biol, 1743. pp. 153-161. ISSN 1064-3745
Abstract
Programmed cell death (PCD) is an essential component of development, biotic and abiotic responses. Hypersensitive response (HR)-associated cell death activated under pathogen attack is one of the most dramatic manifestations of PCD in plants. Signal transduction through mitogen-activated protein kinase (MAPK) cascades, a very conserved signaling pathway across eukaryotes, is a core mediator for HR-associated PCD. Therefore, monitoring MAPK activation enables the mechanisms underlying HR-associated PCD to be elucidated. Here, we describe the use of a phosphorylation-specific MAPK3/6 antibody to monitor the activation of MAPK3/6 during HR-associated PCD. The technique may be adapted for use in other types of PCD.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Hypersensitive response Mapk Phosphorylation Programmed cell death |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation organism description > plant |
| CSHL Authors: | |
| Communities: | CSHL labs > Jackson lab |
| Depositing User: | Matt Covey |
| Date: | January 2018 |
| Date Deposited: | 26 Jan 2018 17:55 |
| Last Modified: | 26 Jan 2018 17:55 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35982 |
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