Isolation of the Cdna-Encoding Rat Skeletal-Muscle Myosin Light Chain Kinase - Sequence and Tissue Distribution

Roush, C. L., Kennelly, P. J., Glaccum, M. B., Helfman, D. M., Scott, J. D., Krebs, E. G. (July 1988) Isolation of the Cdna-Encoding Rat Skeletal-Muscle Myosin Light Chain Kinase - Sequence and Tissue Distribution. Journal of Biological Chemistry, 263 (21). pp. 10510-10516. ISSN 0021-9258

URL: http://www.ncbi.nlm.nih.gov/pubmed/2839493

Abstract

A cDNA clone encoding skeletal muscle myosin light chain kinase (MLCK) was isolated from a rat skeletal muscle library using oligonucleotide probes. The total length of the rat skeletal muscle MLCK cDNA was 2823 base pairs with an open reading frame of 1830 base pairs. The deduced sequence of the 610-amino acid protein exhibited 96% amino acid identity to rabbit skeletal muscle MLCK in the carboxyl-terminal portion of the molecule, which contains the catalytic and the calmodulin-binding domains, and 58% identity in the amino-terminal region. Analysis of total rat mRNA revealed a single mRNA species of 3.4 kilobases that was unique to skeletal muscle. Further analysis of skeletal muscle tissue using fast-twitch glycolytic, fast-twitch oxidative glycolytic, and slow-twitch oxidative fibers isolated from rat leg revealed that the mRNA level for MLCK varied among the three fiber types. The results of kinase assays performed on the fibers showed that MLCK activity levels paralleled the MLCK mRNA levels found in each of the three types of skeletal muscle fibers studied. Fast-twitch oxidative glycolytic (gastrocnemius red) and slow-twitch oxidative (soleus) exhibited 60 and 13%, respectively, of the enzymatic activity present in fast-twitch glycolytic (gastrocnemius white) fibers.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat
CSHL Authors:
Communities: CSHL labs > Helfman lab
Depositing User: Gail Sherman
Date: 25 July 1988
Date Deposited: 15 Sep 2017 15:29
Last Modified: 15 Sep 2017 15:29
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35211

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