Mcra and Mcrb Restriction Phenotypes of Some Escherichia-Coli Strains and Implications for Gene Cloning

Raleigh, E. A., Murray, N. E., Revel, H., Blumenthal, R. M., Westaway, D., Reith, A. D., Rigby, P. W. J., Elhai, J., Hanahan, D. (February 1988) Mcra and Mcrb Restriction Phenotypes of Some Escherichia-Coli Strains and Implications for Gene Cloning. Nucleic Acids Research, 16 (4). pp. 1563-1575. ISSN 0305-1048

URL: http://www.ncbi.nlm.nih.gov/pubmed/2831502
DOI: 10.1093/nar/16.4.1563

Abstract

The McrA and McrB (modified cytosine restriction) systems of E. coli interfere with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected to be sensitive, and this could interfere with cloning experiments. The McrA and B phenotypes of a few strains have been reported previously (1-4). The Mcr phenotypes of 94 strains, primarily derived from E. coli K12, are tabulated here. We briefly review some evidence suggesting that McrB restriction of mouse-modified DNA does occur in vivo and does in fact interfere with cloning of specific mouse sequences.

Item Type: Paper
Subjects: Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

organism description > bacteria > escherichia coli
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: 25 February 1988
Date Deposited: 18 Sep 2017 16:43
Last Modified: 18 Sep 2017 16:43
PMCID: PMC336335
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35200

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