Raleigh, E. A., Murray, N. E., Revel, H., Blumenthal, R. M., Westaway, D., Reith, A. D., Rigby, P. W. J., Elhai, J., Hanahan, D. (February 1988) Mcra and Mcrb Restriction Phenotypes of Some Escherichia-Coli Strains and Implications for Gene Cloning. Nucleic Acids Research, 16 (4). pp. 1563-1575. ISSN 0305-1048
Abstract
The McrA and McrB (modified cytosine restriction) systems of E. coli interfere with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected to be sensitive, and this could interfere with cloning experiments. The McrA and B phenotypes of a few strains have been reported previously (1-4). The Mcr phenotypes of 94 strains, primarily derived from E. coli K12, are tabulated here. We briefly review some evidence suggesting that McrB restriction of mouse-modified DNA does occur in vivo and does in fact interfere with cloning of specific mouse sequences.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment > cloning Investigative techniques and equipment > assays > cloning organism description > bacteria > escherichia coli bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | 25 February 1988 |
| Date Deposited: | 18 Sep 2017 16:43 |
| Last Modified: | 18 Sep 2017 16:43 |
| PMCID: | PMC336335 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35200 |
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