Overexpression of Mouse D-Type Cyclins Accelerates G(1) Phase in Rodent Fibroblasts

Quelle, D. E., Ashmun, R. A., Shurtleff, S. A., Kato, J. Y., Barsagi, D., Roussel, M. F., Sherr, C. J. (August 1993) Overexpression of Mouse D-Type Cyclins Accelerates G(1) Phase in Rodent Fibroblasts. Genes Dev, 7 (8). pp. 1559-1571. ISSN 0890-9369

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URL: http://www.ncbi.nlm.nih.gov/pubmed/8339933
DOI: 10.1101/gad.7.8.1559

Abstract

Mammalian D-type cyclins are growth factor-regulated, delayed early response genes that are presumed to control progression through the G1 phase of the cell cycle by governing the activity of cyclin-dependent kinases (cdks). Overexpression of mouse cyclin D1 in serum-stimulated mouse NIH-3T3 and rat-2 fibroblasts increased their rates of G0 to S- and G1- to S-phase transit by several hours, leading to an equivalent contraction of their mean cell generation times. Although such cells remained contact inhibited and anchorage dependent, they manifested a reduced serum requirement for growth and were smaller in size than their normal counterparts. Ectopic expression of cyclin D2 in rodent fibroblasts, either alone or together with exogenous cdk4, shortened their G0- to S-phase interval and reduced their serum dependency, but cyclin D2 alone did not alter cell size significantly. When cells were microinjected during the G2 interval with a monoclonal antibody specifically reactive to cyclin D1, parental rodent fibroblasts and derivatives overexpressing this cyclin were inhibited from entering S phase, but cells injected near the G1/S phase transition were refractory to antibody-induced growth suppression. Thus, cyclin D1, and most likely D2, are rate limiting for G1progression.

Item Type: Paper
Uncontrolled Keywords: D-TYPE CYCLINS CELL CYCLE G(1) PROGRESSION CDK4 COLONY-STIMULATING FACTOR ONCOGENE V-FMS CELL-CYCLE SACCHAROMYCES-CEREVISIAE G1-SPECIFIC CYCLINS PROTEIN-KINASE GROWTH-FACTOR G1 CYCLIN GENE EXPRESSION
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: August 1993
Date Deposited: 20 Apr 2016 19:07
Last Modified: 03 Nov 2017 20:35
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32511

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