Cyclin G is a transcriptional target of the p53 tumor suppressor protein

Okamoto, K., Beach, D. (October 1994) Cyclin G is a transcriptional target of the p53 tumor suppressor protein. Embo J, 13 (20). pp. 4816-22. ISSN 0261-4189 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7957050

Abstract

Through a PCR-based differential screening method, cyclin G was identified as a novel transcriptional target of the p53 tumor suppressor gene product. In both a mouse p53 temperature-sensitive leukemic cell line and mouse embryonic fibroblasts (MEF) after gamma-irradiation, cyclin G mRNA was rapidly induced. MEF from a p53-deficient mouse expressed cyclin G at a level > 10-fold lower than that from a wild-type mouse. Using a DNA binding assay, a specific p53 binding site was identified upstream from the cyclin G gene, which functioned as a p53-dependent cis-acting element in a transient transfection assay. These results suggest that cyclin G might participate in a p53-mediated pathway to prevent tumorigenesis.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Base Sequence Cells, Cultured Cyclins/biosynthesis/ genetics Mice Molecular Sequence Data Protein Binding Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Trans-Activation (Genetics) Transcription Factors/ physiology Tumor Cells, Cultured Tumor Suppressor Protein p53/ physiology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p53
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > tumor suppressor
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Matt Covey
Date: 17 October 1994
Date Deposited: 24 Aug 2015 14:10
Last Modified: 24 Aug 2015 14:10
PMCID: PMC395420
URI: http://repository.cshl.edu/id/eprint/31425

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