Okamoto, K., Beach, D. (October 1994) Cyclin G is a transcriptional target of the p53 tumor suppressor protein. Embo J, 13 (20). pp. 4816-22. ISSN 0261-4189 (Print)
Abstract
Through a PCR-based differential screening method, cyclin G was identified as a novel transcriptional target of the p53 tumor suppressor gene product. In both a mouse p53 temperature-sensitive leukemic cell line and mouse embryonic fibroblasts (MEF) after gamma-irradiation, cyclin G mRNA was rapidly induced. MEF from a p53-deficient mouse expressed cyclin G at a level > 10-fold lower than that from a wild-type mouse. Using a DNA binding assay, a specific p53 binding site was identified upstream from the cyclin G gene, which functioned as a p53-dependent cis-acting element in a transient transfection assay. These results suggest that cyclin G might participate in a p53-mediated pathway to prevent tumorigenesis.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Animals Base Sequence Cells, Cultured Cyclins/biosynthesis/ genetics Mice Molecular Sequence Data Protein Binding Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Trans-Activation (Genetics) Transcription Factors/ physiology Tumor Cells, Cultured Tumor Suppressor Protein p53/ physiology |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p53 bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > tumor suppressor |
| CSHL Authors: | |
| Communities: | CSHL labs > Beach lab |
| Depositing User: | Matt Covey |
| Date: | 17 October 1994 |
| Date Deposited: | 24 Aug 2015 14:10 |
| Last Modified: | 24 Aug 2015 14:10 |
| PMCID: | PMC395420 |
| URI: | https://repository.cshl.edu/id/eprint/31425 |
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