On-Enzyme Refolding Permits Small RNA and tRNA Surveillance by the CCA-Adding Enzyme

Kuhn, C. D., Wilusz, J. E., Zheng, Y., Beal, P. A., Joshua-Tor, L. (January 2015) On-Enzyme Refolding Permits Small RNA and tRNA Surveillance by the CCA-Adding Enzyme. Cell, 160 (4). pp. 644-658. ISSN 0092-8674 (Public Dataset)

Abstract

Transcription in eukaryotes produces a number of long noncoding RNAs (lncRNAs). Two of these, MALAT1 and Menbeta, generate a tRNA-like small RNA in addition to the mature lncRNA. The stability of these tRNA-like small RNAs and bona fide tRNAs is monitored by the CCA-adding enzyme. Whereas CCA is added to stable tRNAs and tRNA-like transcripts, a second CCA repeat is added to certain unstable transcripts to initiate their degradation. Here, we characterize how these two scenarios are distinguished. Following the first CCA addition cycle, nucleotide binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle. Intriguingly, with the CCA-adding enzyme acting as a molecular vise, the RNAs proofread themselves through differential responses to its interrogation between stable and unstable substrates.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > long non-coding RNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
CSHL Authors:	Joshua-Tor, Leemor Kuhn, Claus-D.
Communities:	CSHL Cancer Center Program > Gene Regulation and Cell Proliferation CSHL Cancer Center Shared Resources > Mass Spectrometry Service CSHL Cancer Center Shared Resources > Proteomics Service CSHL labs > Joshua-Tor lab
Depositing User:	Matt Covey
Date:	27 January 2015
Date Deposited:	09 Feb 2015 15:41
Last Modified:	01 Sep 2017 15:49
PMCID:	PMC4329729
Related URLs:	Publisher
Dataset ID:	PDB: 4X4N PDB: 4X4O PDB: 4X4P PDB: 4X4Q PDB: 4X4R PDB: 4X4S PDB: 4X4T PDB: 4X4U PDB: 4X4V PDB: 4X4W
URI:	https://repository.cshl.edu/id/eprint/31187

Actions (login required)

Administrator's edit/view item