Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation

Shi, S. H., Hayashi, Y., Petralia, R. S., Zaman, S. H., Wenthold, R. J., Svoboda, K., Malinow, R. (1999) Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation. Science, 284 (5421). pp. 1811-6. ISSN 0036-8075 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10364548
DOI: 10.1126/science.284.5421.1811

Abstract

To monitor changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor distribution in living neurons, the AMPA receptor subunit GluR1 was tagged with green fluorescent protein (GFP). This protein (GluR1-GFP) was functional and was transiently expressed in hippocampal CA1 neurons. In dendrites visualized with two-photon laser scanning microscopy or electron microscopy, most of the GluR1-GFP was intracellular, mimicking endogenous GluR1 distribution. Tetanic synaptic stimulation induced a rapid delivery of tagged receptors into dendritic spines as well as clusters in dendrites. These postsynaptic trafficking events required synaptic N-methyl-D-aspartate (NMDA) receptor activation and may contribute to the enhanced AMPA receptor-mediatedtransmission observed during long-term potentiation and activity-dependent synaptic maturation.

Item Type: Paper
Uncontrolled Keywords: Animals Cells, Cultured Dendrites/ metabolism/ultrastructure Electric Stimulation Hippocampus/cytology/physiology Humans Long-Term Potentiation Neuronal Plasticity Neurons/ physiology Organ Culture Techniques Rats Receptor Aggregation Receptors, AMPA/ metabolism Receptors, N-Methyl-D-Aspartate/ physiology Recombinant Fusion Proteins/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Synapses/metabolism/ physiology Synaptic Transmission Tetany
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > Long term potentiation
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission
CSHL Authors:
Communities: CSHL labs > Malinow lab
CSHL labs > Svoboda lab
Depositing User: Kathleen Darby
Date Deposited: 23 Apr 2014 20:27
Last Modified: 23 Apr 2014 20:27
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29824

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